|
KMID : 0861120140180010011
|
|
Korean Journal of Oriental Preventive Medicine
2014 Volume.18 No. 1 p.11 ~ p.21
|
|
|
Effects of fermented Taraxacum mongolicum by Dongchimi on protection of liver cell and immune activity
|
|
Choi Bog-Soo
Kim Hye-Ja
|
|
|
Abstract
|
|
|
Objective : This study set out to combine the treatment efficacy of Taraxacum with Dongchimi fermentationand investigate Taraxacum ’s effects on protection of liver cell and controlling nitric oxide(NO) throughexperiments, thus checking whether it had values as a physiological active matter. The experimental materialsinclude Taraxacum Dongchimi (TD) and Taraxacum fermented by Dongchimi (TDF). As for methodology,experiments were carried out to compare TD and TDF in components, protection effects for liver cells, anticancereffects on liver cells, and protection effects for brain cells in the aspects of liver function andimmunity enhancement.
Method : The experimental materials include Taraxacum Dongchimi (TD) and Taraxacum fermented by Dongchimi(TDF). As for methodology, experiments were carried out to compare TD and TDF in components, protectioneffects for liver cells, anti-cancer effects on liver cells, and protection effects for brain cells in the aspectsof liver function and immunity enhancement.
Results : As shown in the chromatogram results, each valid component content increased in Taraxacumfermented by Dongchimi (TDF) for each time section. Of them, the valid component content at 36.80 minuteswas approximately 2.7 times higher in TDF at 21.8% than in Taraxacum Dongchimi (TD) at 8.28%. TDFgenerated more excellent protection effects against the toxicity that caused oxidative damage to the livercell(HepG2) with t-BHP than TD. The survival rate was low in TD of 100 ㎍/㎖ and 300 ㎍/㎖ and increasedto 23.3% in TDF of 100 ㎍/㎖. The survival rate was the highest at 300 μM with a significant differenceof 68.1%(P<0.05). Both TD and TDF showed effects of controlling nitric oxide production according toconcentration with TDF recording a higher rate of controlling nitric oxide production than TD. There weresignificant differences(P<0.05) in the effects of controlling nitric oxide production at 200 ug/ml, 400 ug/mlin both groups. Especially the result TDF of 400㎍/㎖ was thus similar to those of butein, the positivecontrol group.
Conclusion : The result of this studies is that Taraxacum fermented by Dongchimi (TDF) increased thevalid component content compared with the simple mixture(TD). The findings clearly show that it is a material with the effects of improving immunity and liver cell protection. If fermentation methods are furtherdeveloped to use it as a functional material, it will be subject to more opportunities of being used inother functional foods and make a contribution to integrated medicinal food development
|
|
|
KEYWORD
|
|
|
Taraxacum, Dongchimi, fermentatin, liver cell protection, immune activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|